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Multispectral In Vivo Fluorescence Imaging System

Multispectral In Vivo Fluorescence Imaging System at CSI

Multispectral In Vivo Fluorescence Imaging System at CSI

A new multispectral in vivo fluorescence imaging system (Maestro EX, Cambridge Research & Instrumentation, Woburn, MA) was recently installed at the Center for Systems Imaging (CSI). The Maestro EX system comes equipped with a light-tight enclosure, configurable imaging area, solid-state tunable multispectral camera system, intuitive data acquisition and analysis software, and a computer workstation.

The hardware is supported with industry-leading algorithms incorporated in easy-to-use software. The system contains an optimized liquid crystal tunable filter (LCTF) based multispectral camera system that can capture high spectral resolution multispectral fluorescence images of small animals at multiple wavelengths across the visible to NIR. The system also includes a Xenon-based Illuminator with interference-filter-selectable spectral ranges. An optical heated stage and triple nose-cone anesthesia manifold are available options. A limiting factor for in vivo fluorescence imaging is the confounding signal from the autofluorescence of the animal.

The multispectral imaging system utilizes full multispectral imaging and spectral unmixing algorithms; thus it is able to separate the tissue autofluorescence from the signal of interest, thereby greatly increasing sensitivity and signal-to-noise. The multispectral in vivo fluorescence imaging system could be used to guide the development and evaluation of new drugs, nanoparticles, quantum dots, and imaging probes. Therefore, this device would provide synergy among clinical research involving multiple emphasis areas: imaging, nanotechnology, cancer, the drug discovery, inflammation/vaccines, and animal models of human disease.