Facility Description
Detailed facility information is given below. If you need additional information please contact the Core Director.
Instrumentation
The Emory University School of Medicine Core Facility for Flow Cytometry occupies several locations scattered around the Emory campus. Sorters are located in Emory Hospital and the Vaccine Research Center at Yerkes Primate Research Center. Analyzers are located in Emory Clinic Building B and Woodruff Memorial Building. In addition, support in the form of training, technical assistance, troubleshooting and consultation are given to various laboratories that independently own cytometers on campus. Facility hardware:
Analyzers:
- FACScan (Location: Emory Clinic Building B, Room B3222)
- FACSort (Location: Woodruff Memorial Building, Room 1312A)
- LSRII (Location: Dental School, Room 440A)
The FACScan is the tried and true one-laser, three-color benchtop instrument manufactured by Becton Dickinson. Specifications:
- single laser, 488nm excitation beam wavelength
- three fluorescence detectors:
- FL1: capable of detecting fluorophores and other similar probes in the range of 530nm +/- 15nm.
Examples: FITC (fluorescein iso-thiocyanate), GFP, DCFDA, CFSE (carboxyfluorescein
diacetate succinimidyl ester), and others. - FL2: capable of detecting fluorophores and other similar probes in the range of 585nm +/- 42nm.
Examples: PE (Phycoerythryn), Propidium Iodide, Cy3, and others. - FL3: capable of detecting fluorophores and other similar probes in the range of 670nm.
Examples: Propidium Iodide, CyChrome (PE-Cy5), PerCP (Peridinin chlorophyll protein),
TriColor, PerCP-Cy 5.5, PE-Cy7, and others.
The FACSort is a two-laser, four-color benchtop analyzer manufactured by Becton Dickinson. Specifications:
- primary laser: 488nm excitation beam wavelength
- secondary laser: 635nm excitation beam wavelength
- four fluorescence detectors:
- FL1 - FL3: see FACSCan data above.
- FL4: capable of detecting fluorophores in the range of 661nm +/- 16nm.
Examples: APC (Allophycocyanin)
Sorters:
- FACSVantage (Location: Dental School, room 441)
- MoFlo (Location: Emory Vaccine Center at Yerkes, room 2043)
The FACSVantage is the SE model, manufactured by Becton Dickinson, with hardware for 5-color, high-speed multi-laser sorting. It has 488nm primary lasers and 650nm secondary red lasers and has a UV laser split from the 488 primary. The FACSVantage is located in the Dental School Building, Room 441.
The MoFlo, manufactured by Dako-CytomationCytomation, is located in the Vaccine Research Center. It's capabilities include multi-color (up to 8), high-speed multi-laser sorting. It has a 488nm primary laser, a 650nm secondary laser, and a multi-line third laser that can output UV, red, and violet excitation beams.
All sorters within the facility are capable of single-cell deposition sorting, and all sorts are treated with great care in regards to aseptic techniques, accuracy in post-sort recovery and high-purity (>98%). The Moflo is capable of 50,000+ events/second sort rates, and duplicates the purity and recovery data of the FACSVantages.
Personnel
Robert Karaffa, Technical Director (rkaraff@emory.edu)