Facility Description
Detailed facility information is given below. If you need additional information please contact the Core Director.
Instrumentation
The Emory University School of Medicine Core Facility for Flow Cytometry occupies several locations scattered around the Emory campus. Sorters are located in the Dental School Building. Analyzers are located in the Dental School Building and Emory Clinic Building B. In addition, support in the form of training, technical assistance, troubleshooting and consultation are given to various laboratories that independently own cytometers on campus. Facility hardware:
Analyzers:
- FACSort (Location: Emory Clinic Building B, Room B3222)
- LSRII (Location: Dental School, Room 440A)
The FACSort is a two-laser, four-color benchtop analyzer manufactured by Becton Dickinson. Specifications:
- primary laser: 488nm excitation beam wavelength
- secondary laser: 635nm excitation beam wavelength
- four fluorescence detectors:
- FL1: capable of detecting fluorophores and other similar probes in the range of 530nm +/- 15nm.
Examples: FITC (fluorescein iso-thiocyanate), GFP, DCFDA, CFSE (carboxyfluorescein
diacetate succinimidyl ester), and others. - FL2: capable of detecting fluorophores and other similar probes in the range of 585nm +/- 42nm.
Examples: PE (Phycoerythryn), Propidium Iodide, Cy3, and others. - FL3: capable of detecting fluorophores and other similar probes in the range of 670nm.
Examples: Propidium Iodide, CyChrome (PE-Cy5), PerCP (Peridinin chlorophyll protein),
TriColor, PerCP-Cy 5.5, PE-Cy7, and others. - FL4: capable of detecting fluorophores in the range of 661nm +/- 16nm.
Examples: APC (Allophycocyanin)
The LSRII is a three-laser, eleven-color digital benchtop analyzer manufactured by Becton Dickinson.
Specifications:
- primary laser: 488nm (blue) excitation beam wavelength
- secondary laser: 635nm (red) excitation beam wavelength
- tertiary laser: 405nm (violet) excitation beam wavelength
- eleven fluorescence detectors (for more information please see our "Instructions" page, "Training Materials" section)
Sorters:
- FACSVantage (Location: Dental School, room 441)
- FACSAria II (Location: Dental School, room 441)
The FACSVantage is the SE model, manufactured by Becton Dickinson, with hardware for 5-color, high-speed multi-laser sorting. It has 488nm primary lasers and 650nm secondary red lasers and has a UV laser split from the 488 primary. The FACSVantage is located in the Dental School Building, Room 441.
The FACSAria II is manufactured by Becton Dickinson, with hardware for 11-color, high-speed multi-laser sorting. It has a 488nm primary laser, 635nm secondary red laser and 405nm violet third laser, with capabilities of sorting up to 4 populations at one time. The FACSAria II is located in the Dental School Building, Room 441
All sorters within the facility are capable of single-cell deposition sorting, and all sorts are treated with great care in regards to aseptic techniques, accuracy in post-sort recovery and high-purity (>98%).
Personnel
Robert Karaffa, Technical Director (rkaraff@emory.edu)
Ms. Sommer Durham, M.Sc., Flow Cytometry Technologist Senior