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Synthetic Oligonucleotide Order Form
Requester:
Please double check the information below and remember to enter your account number.
Contact Name: Date:
Dept: Phone:
Bldg: FAX:
PI Name: Acct#:
E-mail Address:

Instructions:

  1. Provide sequence name.
  2. Select synthesis scale.
  3. Select purification.
  4. Select 5'-OH modification.
  5. Enter in your sequence using a slash or space after each triplet. Use the special codes listed below for mixed sites.Please do not press enter at the end of each line.
  6. Select 3'-OH modification.
  7. Enter in the total number of bases.
  8. Select additional modifications.
  9. Enter any comments or special instructions.

Sequence Name: (limit 8 characters)

Synthetic Scale:
30-40 nmol (~8-16 µg/base)
0.2 µmol (~33 µg/base)
1.0 µmol (~160 µg/base)
2.5 µmol (~240 µg/base)
10 µmol (~1.65 mg/base)

Purification:
None
RP-HPLC


Single-letter base coding:

A=A    C=C    T=T    G=G     M=AC   R=AG    W=AT
S=CG   Y=CT   K=GT   V=ACG   H=ACT  D=AGT   B=CGT   N=ACGT
I=deoxyinosine
5'-OH Sequence*
(Please DO NOT hit the enter key to end a line.)
 3'-OH
     
*Please use single letter codes for mixed sites. Special bases should be identified in the comments section below as well in the "Other" field under the base composition below.



Oligo Length: Molecular Weight: **
**Based on sequence entered above with hydroxl on 5' and 3'.


Number of bases in oligo:
A C G T
M=AC R=AG W=AT S=CG
Y=CT K=GT V=ACG H=ACT
D=AGT B=CGT N=ACGT    
I=deoxyinosine Other1 Other2 Other3


Please define "OtherX" in the comments section below.

Other Modifications:
Please indicate any special instructions for the modifications, i.e. placement of site-specific modifications, in the comments section.

Phosphothioate: All Site-specific
Fluorescent dye labeling: 6-FAM, HEX, TET, TAMARA (3' or 5'),
ROX, CY5
Blocking of 3'-OH against polymerase extension
C-5 propyne analogs of dC and dT for antisense experiments
Addition of deoxyuridine

Comments:

Last updated by Matt Reed. 11/13/2003